e. coli strain bl21-codonplus (de3)-ril (Agilent technologies)
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e. coli strain bl21-codonplus (de3)-ril
E. Coli Strain Bl21 Codonplus (De3) Ril, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+strain+bl21-codonplus-ril/pmc10936432-279-12-17
Average 90 stars, based on 1 article reviews
E. Coli Strain Bl21 Codonplus (De3) Ril, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+strain+bl21-codonplus-ril/pmc10936432-279-12-17
Average 90 stars, based on 1 article reviews
e. coli strain bl21-codonplus (de3)-ril - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Botulinum neurotoxin X lacks potency in mice and in human neurons"
Article Title: Botulinum neurotoxin X lacks potency in mice and in human neurons
Journal: mBio
doi: 10.1128/mbio.03106-23
Figure Legend Snippet: Characterization of recombinant BoNT/X expressed in E. coli . (a) Coomassie stained SDS-PAGE gel of BoNT/X expressed in E. coli BL21 (DE3), purified via IMAC-Ni 2+ column, and treated with LysC or trypsin at 250:1 (wt/wt) BoNT/X:LysC or trypsin. R, reduced (with 100 mM DTT); NR, non-reduced. (b) In vitro activity of rBoNT/X in hiPSC-derived neuronal cell lysates. Cell lysates were incubated with either trypsinized or non-trypsinized BoNT/X in reducing buffer and were analyzed via western blot for VAMP2 cleavage. Syntaxin was used as a loading control. (c) In situ activity of rBoNT/X in hiPSC-derived neurons. Cultured neurons were exposed for 24 hours to serial dilutions of BoNT/X, and cell lysates were analyzed for VAMP2 cleavage via western blot. Estimated concentrations (nM) of BoNT/X are noted. (d) Antibody neutralization cell assay. Trypsin-treated BoNT/X (20 nM) was incubated with anti-BoNT/X antiserum for 1 hour, and hiPSC-derived neurons were exposed to the antibody-BoNT/X mixture, BoNT/X alone, anti-BoNT/X antiserum alone, or culture media alone for 40 hours. Cell lysates were analyzed for VAMP2 cleavage via western blot, using syntaxin as a loading control.
Techniques Used: Recombinant, Staining, SDS Page, Purification, In Vitro, Activity Assay, Derivative Assay, Incubation, Western Blot, In Situ, Cell Culture, Neutralization
Figure Legend Snippet: Native BoNT/X causes mild local paralytic effects on mice. (a) Mice ( n = 3) were injected into the left gastrocnemius muscle with either 2.0 μg BoNT/X complex or 10 pg BoNT/B (Okra). The right hindlimb was injected with vehicle. Shown are representative images of digital abduction at 24 hours post-injection, where both BoNT/X and BoNT/B complexes result in moderate muscle paralysis. (b) Reduction in reflexive spread of digits was quantified via the DAS, with average values and standard deviations depicted in the graph ( n = 3). (c) Confocal microscopy depicting the lack of native BoNT/X binding to neuronal PC12 (rat) cells. (d) Minimal lethal dose (MLD) of recombinant X(LC-HC N )/A(HC C ) chimera, expressed in E. coli . Estimated concentration BoNT/(X/A1) holotoxin is indicated based on densitometry of total purified protein.
Techniques Used: Injection, Confocal Microscopy, Binding Assay, Recombinant, Concentration Assay, Purification
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